In order to examine the effect of a V. anguillarum infection on immune-related gene expression early upon infection, we compared the tlr3, tlr5m, cas1, il1β, tnfα, mif , il10, cc1, cxcl8, cxcr, ccr9, hpc, and dic gene transcript levels for groups C2 (no MP, infected) and C3 (no MP, non-infected) at 18, 24, and 36 h p.i. The mean gene transcript levels ± SD for group C2 relative to group C3 are presented in Figure 4. The gene discussed is TNF; the disease is infection.