Cytokine production of GM-CSF, TNF-α, MIP-1α, and IFN-γ of lentiviral/RD114-TR CD19-CAR-NK cells was analyzed 3 days after transduction upon expansion in low dose IL-15 alone and in context of co-culturing with target-specific Sup-B15 ALL cells at an E:T ratio of 1:1 for 4 h. This evidence concerns the gene CD19 and acute lymphoblastic leukemia.