Considering the alteration of cell number after virus infection and necrostain-1 treatment, before calculating intracellular and extracellular viral genome level and TCID50, the cell number was detected, and it was found that different dose of necrostatin-1 (Supplementary Figure S2A) and necrostatin-2 (Supplementary Figure S2B) could prevent the damage induced by CA6 infection, but not caspase-3 inhibitor (Supplementary Figure S2C). Here, CASP3 is linked to viral infectious disease.