In line with the alteration of DNA methylation, gene expression analysis using qPCR showed reduced mRNA expression of Wnt5a and increased mRNA expression of Pik3c2a, Pik3c2b, and Pik3r2 in mice exposed to HF-inulin compared with mice in the HF group (P < 0.01, Figures 5B,D,F,H). Here, PIK3C2B is linked to hydrops fetalis.