Consistent with the methylation array results, the BSP results showed that Wnt5a gene methylation was decreased (P < 0.01, Figure 5A), while Pik3c2a, Pik3c2b, and Pik3r2 gene methylation was increased in mice in the HF group (P < 0.01, Figures 5C,E,G). Here, PIK3C2B is linked to hydrops fetalis.