To further explore the anti-inflammatory mechanism of baicalin in cholestasis rats, we examined the protein expression level of NF-κB, which is considered a “master switch” in the regulatory mechanism of inflammatory processes that can regulate numerous inflammatory factors, including IL-6, IL-1β, and TNF-1α (Liu et al., 2017; Zhang J et al., 2018). This evidence concerns the gene NFKB1 and cholestasis.