In this study, we found that MA exposure significantly increased the total and differential leukocyte count in the airways of mice, especially in females with prominent changes in ERβ KO mice which concurs with clinical evidence (Melgert et al., 2005; Blacquiere et al., 2010; Fuseini and Newcomb, 2017; Pignataro et al., 2017; DeBoer et al., 2018) and our own pre-clinical findings (Aravamudan et al., 2017; Ambhore et al., 2018; Ambhore et al., 2019a) indicating females are more susceptible to asthma and that ERβ plays a protective role in regulating inflammatory cell infiltration. This evidence concerns the gene ESR2 and asthma.