In the immunohistochemistry and immunofluorescence analyses, MKK4, MKK7, c-Jun and caspase-3 expression was obviously higher in the AD group than in the N group, but this expression decreased after the intervention in the AD + SP, AD + EA and AD + SP + EA groups (Figures 5A–J2, 6A–J2). Here, MAP2K4 is linked to Alzheimer disease.