To determine whether endothelial dysfunction can contribute to CBF dysregulation as seen in TAM-treated pericyte-CreER; iDTR mice after DT treatment, we tested CBF response to acetylcholine, an endothelium-dependent receptor-mediated vasodilator (Figure 1G; Kisler et al., 2017b). The gene discussed is CEBPZ; the disease is endothelial dysfunction.