The present study confirmed that intrathecal injection of methyllycaconitine in the rat model of bone cancer pain blocked spinal cinobufagin-induced mechanical antiallodynia and pretreatment with methyllycaconitine in cultured primary microglial cells attenuated cinobufagin-stimulated gene expression of IL-10 and POMC. The gene discussed is IL10; the disease is bone neoplasm.