Furthermore, treatment with the specific α7-nAChR agonist PHA-543613 in cultured primary microglia stimulated gene and protein expression of IL-10 and β-endorphin, and intrathecal PHA-543613 in bone cancer pain rats produced mechanical antiallodynia, both of which were blocked by the pretreatment with methyllycaconitine. This evidence concerns the gene CHRNA7 and bone neoplasm.