The present study confirmed that intrathecal injection of methyllycaconitine in the rat model of bone cancer pain blocked spinal cinobufagin-induced mechanical antiallodynia and pretreatment with methyllycaconitine in cultured primary microglial cells attenuated cinobufagin-stimulated gene expression of IL-10 and POMC. This evidence concerns the gene POMC and bone neoplasm.