The data showed that compared with PBS group, α-melittin-NPs induced increases in the frequencies of IFN-γ+CD8+ (12.2-fold) and IFN-γ+CD4+ (7.2-fold) T cells at day 21 after tumor implantations (Fig. 5a–c) but no differences emerged at 14 days (Supplementary Fig. 13a, b). This evidence concerns the gene CD8A and neoplasm.