These results were contrast to that in HCT116 cells, in which the binding of H3K27Ac in the Runx2 promoter was increased by knocking down CBX412, indicating that the opposite effects of CBX4 on Runx2 between osteosarcoma cells and CRC cells may be due to the different status of H3K27Ac in the Runx2 promoter. The gene discussed is CBX4; the disease is osteosarcoma.