To evaluate the clinical significance of the regulation of CBX4 by CK1α, the immunohistochemical (IHC) staining using both anti-CBX4 and anti-CK1α antibodies, which are highly specific12 (Supplementary Fig. 9), were performed in 55 osteosarcoma tissues (Supplementary Data 1). Here, CSNK1A1 is linked to osteosarcoma.