Flow cytometric analysis of mouse TI CD8+ cells isolated from various cancer models, including MC38 colon cancer, CT26 colon cancer, TC-1 lung cancer, and LLC1 lung cancer, revealed a similar correlation between TOX expression and TI CD8+ T cell exhaustion severity (Additional file 2: Figure S3 and S4A). The gene discussed is TOX; the disease is malignant colon neoplasm.