While a predominant basolateral plasma membrane (PM) staining similar to +/+ #1 was detected in three out of six BD-associated (+/N11K, +/R218C, +/A243V) and two ADVIRC cell lines, BD-associated cell lines +/I295del #1 and #2 and particularly +/Q238R demonstrated a large fraction of intracellularly retained mutant BEST1 protein. Here, BEST1 is linked to Behcet disease.