Herein, for the first time, we report that while 5mC distribution in the CXCR4 promoter was not significantly changed in DNA from primary colon cancer tissue (compared to control tissue DNA), we observed that enhanced CXCR4 expression in CRC associates with increased 5hmC deposition in the gene body. This evidence concerns the gene CXCR4 and malignant colon neoplasm.