To investigate 5mC methylation in CXCR4 in human colon cancers, we extracted DNA from tumor colonocytes and adjacent normal-appearing colonocytes and tested DNA for the presence of 5mC in 3 CXCR4 genomic loci by COBRA as illustrated in Supplementary Figure S2. This evidence concerns the gene CXCR4 and malignant colon neoplasm.