Further stratification studies showed that age at diagnosis, race, gender, tumor differentiation, SEER historic stage, tumor size, lymph node status, surgical intervention (including local tumor destruction and surgery), AFP level, and fibrosis score had a significant impact on the OS of Klatskin tumor, ICCA, and HCC patients (p < 0.05) (Fig. 3). The gene discussed is AFP; the disease is neoplasm.