To determine whether the GEFD1 and spectrin variants affected these processes, we transfected N1E-115 neuroblastoma cells with the different TRIO mutants and quantified neurite outgrowth (Figures 4C and 4E) and lamellipodia formation both along the shaft and at the tip of the neurite (Figures 4D and 4E). Here, TRIO is linked to neuroblastoma.