Following the increase in body mass, the HF diet also induced MS in mice, corroborating our previous published work demonstrating that this duration and composition of HF diet results in obesity, glucose, and insulin intolerance, increased fasting blood glucose and insulin levels, raised blood cholesterol and low density lipoproteins (LDL) cholesterol concentrations, and elevated homeostatic model assessment of insulin resistance (HOMA‐IR), thus characterizing the MS (de Sá et al., 2016). The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.