In our previous studies, two poorly differentiated hepatoma subpopulations, Huh‐7‐trans and Huh‐7‐DN with aberrant Hh activation, were isolated from their parental Huh‐7 cells by fluorescence‐activated cell sorting (FACS) with a negative CD133/EpCAM surface marker profile. The gene discussed is PROM1; the disease is hepatocellular carcinoma.