It is apparent from the heat map of the verified data set that major differentially expressed genes that are categorized into Hh (GLI1, GLI2), Wnt (WNT5B) or Hippo signalling (FGF1, TGFB1) are involved in the modulation of cancer progression and were up‐regulated, indicating that Huh‐7‐trans and Huh‐7‐DN cells might be more aggressive than parental Huh‐7 cells. This evidence concerns the gene WNT5B and cancer.