SHH and infection: Ten weeks after infection of KrasLox-Stop-Lox-G12D/+;Trp53fl/fl (KP) mice [47] with adenovirus-expressing cre recombinase (adeno-cre) by intranasal inhalation, LAD expressed Shh mRNA as shown by in situ hybridization (Fig. 2a, Supplementary Fig. 5b).