It’s consistent with that combination of nontoxic OTA (0.25 mg/kg per day, qod) and low dosage of CsA (20 mg/kg per day) could induce renal fibrosis by upregulating expressions of Vimentin, TGF-β1, and α-SMA what is same as the conventional high dosage of chronic CsA model (30 mg/k per day), but the individual CsA (20 mg/kg per day) did not induce. The gene discussed is ACTA1; the disease is renal fibrosis.