In order to confirm the myogenic induction of hSkMCs, we have performed, using Real-Time qPCR, the analysis of the MRFs (MyoD-1, Myf-5, MRF-4, Myogenin), the main myogenic differentiation genes, Desmin and MHC, after cultivating cells in MM up to 9 days. The gene discussed is DES; the disease is Miyoshi myopathy.