MYC and non-small cell lung carcinoma: To investigate the function of YAP and ALKBH5 in NSCLC cells, we then used siRNAs and shRNAs to knock down YAP (siYAP-1 and siYAP-2) and ALKBH5 (shALKBH5–1 and shALKBH5–2), or overexpressed these genes by transfecting A549 and H1299 cells with a plasmid encoding Myc-YAP or Flag-ALKBH5, accordingly (Fig. 1f, Additional file 2: Fig. S1d, e).