Using lung healthy cells and tumor cells, we demonstrated the following: (1) ALKBH5 decreases the level of m6A YAP mRNA modification; (2) YTHDF1 and YTHDF2 competitively bind YTHDF3 in an m6A-independent manner to regulate YAP expression; (3) YTHDF2 facilitates the decay of YAP mRNA, which is mediated by Argonaute 2 (AGO2) system and regulated by m6A modification; (4) YTHDF1 promotes m6A modification-mediated YAP mRNA translation via interaction with eIF3a; (5) ALKBH5 decreases the activity of YAP by regulating the miR-107/LATS2 axis in an HuR-dependent manner. The gene discussed is ALKBH5; the disease is neoplasm.