Next, to investigate the function of YTHDF1 in the occurrence and development of NSCLC, we overexpressed YTHDF1 by transfecting A549 and H1299 cells with pcDNA 3.1-YTHDF1, and knocking down of YTHDF1 by siYTHDF1–1 and siYTHDF1–2 (knocking down YTHDF1 using siYTHDF1–2 was more effective than siYTHDF1–1, so siYTHDF1–2 was used to carry out then subsequent experiments) in A549 and H1299 cells (Additional file 9: Fig. S8d, e). Here, YTHDF1 is linked to non-small cell lung carcinoma.