Subsequent direct tests involving 10 prioritized genes (ARL2, BUB3, DSN1, NUF2, SPC24, GART, SHMT2, GARS, PIGS and SKP1) encoding a diverse array of biological functions and exhibiting frequent gene copy number losses and links to worse patient outcomes were performed that validated each as a novel CIN gene. This evidence concerns the gene BUB3 and cervical squamous intraepithelial neoplasia.