Of note, K-563 abrogated the expression of NRF2 target genes such as GCLC, GCLM, AKR1C1 (reductase family 1 member C1), ME1, NQO1 and TXNRD1 in A549 NSCLC cells and in the human gallbladder cancer (GBC) cell line TGBC24TKB, without altering the NRF2 protein levels, its ARE-binding ability, or its nuclear localization. This evidence concerns the gene NFE2L2 and non-small cell lung carcinoma.