Nevertheless, according to genotype, a significantly higher proportion of virus-carrying ≥3 substitutions in ISDR and ≥6 in PKR-bd was observed in patients who developed HCC infected with genotype 2c (5/5, 100%) than with patients infected with genotype 1b (2/8, 25%) (p < 0.001). This evidence concerns the gene EIF2AK2 and hepatocellular carcinoma.