The eCBome is important in the regulation of gut permeability since blocking CB1 activity in mice fed with obesogenic diet inhibited the development of obesity, improved glucose homeostasis and ameliorated intestinal permeability by lowering circulating LPS levels and inflammatory cytokine profile in parallel with enhanced Akkermansia muciniphila and decreased Lachnospiraceae in the gut [29]. This evidence concerns the gene CNR1 and obesity due to melanocortin 4 receptor deficiency.