In this study, we investigated the analgesic effect of the combination of B1/B6/B12 (VBC) and its effects on the expression of P2X3 and TRPV1 receptors in DRG neurons and the inflammatory cytokines IL-1β, TNF-α, and NGF in the spinal cord in STZ-induced diabetic rats. This evidence concerns the gene P2RX3 and diabetes mellitus.