Furthermore, dual immunofluorescence results revealed that CD226 and Mac-3 co-localization was commonly observed in the infarct and border zone, and CD226+Mac3+ macrophages accounted for around 74% of the CD226-expressing cells in the infarcted heart at day 7 after MI (Figure 1C-E and Figure S1C). Here, CD226 is linked to myocardial infarction.