SIRT2 and non-small cell lung carcinoma: Consistently, immunofluorescence (IF) staining of endogenous AKR1C1 and SIRT2 revealed their co-localization (preferentially in cytoplasm) in NSCLC NCI-H460 cells (Figure 4D) harboring high levels of SIRT2 and AKR1C1 (Figure S4A).