To replicate the findings that acetylation of AKR1C1 is fundamental for the metastasis of NSCLC in vivo, H&E staining was used to evaluate tumor metastasis in nude mice intravenously injected with NCI-H1299 cells stably expressing Vector, AKR1C1-WT and AKR1C1-2KR (Figure S3B). Here, AKR1C1 is linked to non-small cell lung carcinoma.