In summary, there is a necessity for standardization of glioma stem cell culture to prevent biased results caused by the medium components and we propose a standard glioma stem cell culture media, which should include neurobasal medium with N2 (Gibco), EGF, bFGF, leukemia inhibitory factor, and heparin to avoid discrepancies in the results of glioma stem cell studies due to technical culture issues. The gene discussed is LIF; the disease is central nervous system cancer.