Interpretation of qPCR data: To determine the relative expression of E-Cadherin in samples, the copy numbers of E-Cadherin molecules present in each sample were pooled into four groups: Group 1 (Hyperorthokeratosis / Hyperparakeratosis), Group 2 (Mild dysplasia), Group 3 (Moderate dysplasia) and Group 4 (Severe dysplasia). Here, CDH1 is linked to dysplasia.