The analysis of protective efficacy in different mice groups after infection with virulent Brucella abortus 544 showed that Omp25+L7/L12 immunized mice exhibited a significant increase in log10 protection (1.98) as compared to the control (i.e. alum immunized mice) at 28 days after challenge (p value < 0.001; Table 1). The gene discussed is SYNJ2BP; the disease is infection.