To test whether HSV-1 activation of the inflammasome in macrophages requires entry and infection through known mechanisms, MDMs pretreated with IFNγ were inoculated with HSV-1 mixed with neutralizing human monoclonal antibodies directed against the HSV-1 glycoprotein D, which is required for HSV-1 entry [37]. The gene discussed is ACKR1; the disease is infection.