More importantly, the changed level of apoptosis and proliferation in pLKO.1‐POU6F2‐AS2 +5‐FU treatment was larger than the sum of the other two groups, which indicating down‐regulated lncRNA POU6F2‐AS2‐enhanced sensitivity of 5‐FU by decreasing the expression of cancer resistance‐related gene. This evidence concerns the gene POU6F2 and cancer.