The investigation was planned based on PPI network analysis which showed that GAPDH, PRDM10, TP53, AKT1, INS, ALB, SRC, MAPK1, ACLY, ACACA, DECR1, ACACB, MBOAT4, TNF, EHHADH and JUN genes are the important genes in progression of NAFLD through the high fat diet treatment (12). This evidence concerns the gene AKT1 and metabolic dysfunction-associated steatotic liver disease.