VIRMA and metastatic neoplasm: Using Bayesian hypothesis testing and deepSNV, the significance of each mutation frequency was assessed.29 Upon analysis, all VAFs were reported significant by deepSNV in metastatic tumours relative to the matched primary tumours, apart from a mutation of KIAA1429 gene in patient OVA_013 and a mutation of BEGAIN gene in patient OVA_048 (Supplementary Table S5, p < 0.05).