All protein coding exons of 26 additional cancer-associated genes were analyzed by NGS including: AKT1, ALK, AR, BRAF, DDR2, EGFR, ERBB2, FGFR3, GNA11, GNAQ, GNAS, IDH1, IDH2, KIT, KRAS, MET, NRAS, PDGFRA, PIK3CA, PTEN, RET, SDHA, SDHB, SDHC, SDHD and TP53. In 31 out of 204 (15.2%) DTF cases, additional non-CTNNB1 alterations were detected (summarized in Fig. 3A,B and Supplementary Tables S1 and S2). The gene discussed is PTEN; the disease is cancer.