In addition, overlapping peptides derived from some random selected proteins (tubulin β-4b chain, heterogeneous nuclear ribonucleoprotein A1, glyceraldehyde-3 phosphate dehydrogenase) that were shown to be downregulated in CDDP-ap NSCLC cells, and upregulated in their live cell counterparts in the form of entire proteins, were also tested (Supplementary Fig. 11a): no significant CD8+ or CD4+ T cell responses against these peptides were shown in six patients analyzed, as compared with HDs (Supplementary Fig. 11b–e). The gene discussed is CD4; the disease is non-small cell lung carcinoma.