To validate the relationship between proteins identified by SILAC-based proteomics and NSCLC, immunohistochemical (IHC) analyses were performed in cancer tissues derived from various patients, by using (commercially) available specific mAbs that were obtained by immunization with the proactivator polypeptide (PSAP)325–524, or the N terminal domain of LYRIC sequences. The gene discussed is MTDH; the disease is cancer.