To test this, we quantified DC trafficking after dorsal MLV ablation by intratumoral injection of 0.5 μm FITC-labeled beads that are too large to flow into LVs and instead must be taken up by DCs in the tumor before being transported to dCLNs.41 As expected, we found that CD11c+MHCII+FITC+ cells in the dCLNs were dramatically reduced in dorsal MLV-defective mice (Supplementary information, Fig. S7a; Fig. 3d), suggesting that dorsal MLVs are critical for the trafficking of DCs to dCLNs. This evidence concerns the gene ITGAX and neoplasm.