Consistently, western blot of kidney tissues showed that overexpression of S100A16 in Tg mice promoted the expression of renal fibrosis-related proteins including fibronectin, α-SMA, and collagen I proteins (Fig. 2c–f), in contrast, S100A16 knockout significantly attenuated their expressions (Fig. 2g–j). Here, FN1 is linked to renal fibrosis.