Subsequently, many studies had investigated the roles of PNPLA3 I148M and TM6SF2 E167K in different countries and ethnic, and the results also proven that PNPLA3 I148M and TM6SF2 E167K variants were the risk factors for the development of NAFLD [10–13]. The gene discussed is TM6SF2; the disease is metabolic dysfunction-associated steatotic liver disease.