In the present study, 12 protein kinases including EGFR, HER2, HER3, HER4, VEGFR3, VEGF-C, EphA3, EphrinA1, p-Akt1, Akt1, beta-catenin and Wnt5a were examined in CCA tissues obtained from190 cases using IHC. The gene discussed is ERBB4; the disease is cholangiocarcinoma.