In the current study, we hypothesized that the alteration of these protein kinases including EGFR family, VEGFR3 and its ligand, Eph receptor and its ligand, Akt1 and its activated form, Wnt, and beta-catenin may be used as the predicting markers for post-operative CCA recurrence. The gene discussed is CTNNB1; the disease is cholangiocarcinoma.