EPCAM and inflammatory bowel disease: To confirm this finding, we generated both HIOs and HCOs from one control line and the two VEO-IBD lines, dissociated them to a single cell suspension, positively selected for EpCAM+ cells via MACS and cultured them either in a three dimensional matrix to generate epithelial only- HIOs (eHIOs) and HCOs (eHCOs) or as monolayers in Transwell inserts.