By RT-PCR with primer pair specific for intron 13 (Figure 1a, Table 1), we amplified HIPK2-S mRNA in human cells that express HIPK2-FL and HIPK2-∆e8 isoforms, such as immortalized human fibroblasts (HFs), freshly isolated peripheral blood mononuclear cells (PBMCs), and HCT116 colon cancer cells (Figure 1d). Here, HIPK2 is linked to malignant colon neoplasm.