To explore the potential function of MAPK signaling in stiffer ECM-induced YAP activation, we treated HCC cells cultured on different stiffness of hydrogels with an MEK1/2 inhibitor (U0126), a JNK inhibitor (SP600125), or a p38 kinase inhibitor (SB203580). This evidence concerns the gene MAPK8 and hepatocellular carcinoma.