TWIST1 and gastric cancer: Therefore, we performed western blot analysis of key proteins involved in the Wnt/β-catenin signaling pathway, such as, β-catenin, Wnt2, GSK-3β, p-GSK-3β, NF-κB p65, Snail1, Slug, and TWIST) in SERPINH1-overexpressing and -silenced GC cells.