The isolated EVs were lysed and measured by ELISA, and significant differences of optical density were observed between PCa and healthy donors among PSA, PSMA, epithelial cell adhesion molecule (EpCAM), heat shock protein 90 (HSP90) and epidermal growth factor receptor 1 (EGFR1) (Figure 4B). This evidence concerns the gene EPCAM and posterior cortical atrophy.