Another study by Bryzgunova et al. demonstrated that small EVs (based on size ranging 30-100 nm) isolated from both healthy donors and PCa patients count to 95% and 90% of total EVs respectively using 100 nm UF membrane, and the isolated samples are positive to EV biomarkers CD63, CD9 and CD24 25. The gene discussed is CD63; the disease is posterior cortical atrophy.