In summary, our in vitro study provides novel evidence that F. alocis upregulates COX2 at both transcriptional and protein levels through TLR2 and MAPK signaling mechanisms in human gingival fibroblastic and monocytic cells, suggesting that F. alocis may play a role in periodontal disease initiation and progression, therefore contributing to periodontal inflammation and tissue destruction. This evidence concerns the gene TLR2 and periodontal disorder.