Thus, overall, we have simplified the amplification of AD tau aggregates by two methods: (1) by modifying the ionic species present in the reaction mixture of the original AD tau RT-QuIC [23] and (2) by extending the τ306–378 fragment N-terminally to include R1, and C-terminally to residue 400 in this work. The gene discussed is MAPT; the disease is Alzheimer disease.