In patient C216, who had been undergoing anti-VEGF treatment for 9 months, we detected the emergence of the 13q12.2 amplification which correlated with the development of progressive disease and resistance to bevacizumab, i.e., increase in blood CEA and CA19-9 tumor markers and increase in lesion size of liver metastases, as observed in the CT scan (Fig. 4; Additional file 3: Table S2; Additional file 2: Figure S5). Here, VEGFA is linked to neoplasm.